Anticancer potentials of peptides of coelomic fluid of earthworm eudrilus eugeniae ms dinesh 1 pharmaceutical effects of earthworms has been apoptosis of hela cells in vitro ( yanqin, yan, zhenjun, shijie, chong, yan, yuhong, 2007) cytotoxic effect of coelomic fluid of earthworm eudrilus eugeniae was studied on baby. The worm was placed in a petri dish in an electrolyte solution (01% nacl) that also served to collect the extruded coelomic fluid (ecf), and a potential difference of 9 v applied across the worm for 20–30 s. The endogenous metabolites of the coelomic fluid of the earthworm eisenia veneta were characterised using high-resolution one-dimensional and two-dimensional 1h nuclear magnetic resonance spectroscopy signals from common organic acids, such as acetate, fumarate, malonate, malate, formate, and. Hence, it can be concluded that the earthworm population has got its influence on soil fertility the more the number of earthworms, more availability of coelomic fluid, which indirectly influences the fertility of the soil, enhances the economy of the nation and the world at large. Coelomic fluid of earthworms have caused much attention in many scientists (cooper, 2002 yu shen, 2010)  researchers have already investigated some features and activities of few enzymes that were isolated and purified from coelomic fluid.
Coelomic fluid the fluid inside the coelom is known as coelomic fluid this is circulated by mesothelial cilia or by contraction of muscles in the body wall which are themselves of mesin [ clarification needed ] [4. The coelomic fluid of earthworms lampito mauritii and megascolex konkanensis have high antimicrobial activity when compare to drawida impertusa and drawida lennora against aeromonas hydrophila, bacillus. Earthworm, dilong in chinese, is a traditional chinese medicine used to treat many diseases including tumors to investigate whether the coelomic fluid of the earthworm eisenia fetida inhibits. Results results showed that liquid vermicompost, coelomic fluid and vermiwash had the greatest effect on egg hatching inhibition, respectively, and coelomic fluid, vermiwash and liquid vermicompost had the highest effect on mortality of larvae (j 2), respectively, in vitroall earthworm-based products were added to the cucumber pots and then a root-knot disease as well as plant growth indices.
The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. Effects of metals on nonspecific immune functions of earthworm based on coelomic fluid lysozyme activity and oxidative antimicrobial activity of coelomic phagocytes phagocytic activity was measured by a reduction of nitroblue tetrazolium (nbt) dye. Coelomic fluid from eudrilus eugeniae was extracted by cold shock treatment bhk21 cells were cultured according to standard cell culture method bhk21 cells were treated with the various protein concentrations of coelomic fluid of eudrilus eugeniae to study the cytotoxoc effect. Total coelomic fluid of earthworms eisenia foetida (oligochaeta, annelida) is capable of lysing different mammalian tumor cell lines this cytolytic activity is different from tumor necrosis factor (tnf)-mediated lysis and is not due to proteolysis.
11 bacteria have been isolated from the coelomic fluid and medium of eisenia foetida and the effect of the coelomic fluid on them assayed22. The coelomic fluid of the earthworm eisenia fetida andrei has antimicrobial activity against aeromonas hydrophila and bacillus megaterium, both known earthworm pathogens (10,11) another aspect of earthworm innate immunity is the phagocytic activity of synergistic effects have been investigated with respect to resistance research has. The first line of innate immunity in earthworms is the body wall that prevents the entrance of microbes into the coelomic cavity that contains fluid in which there are numerous leukocyte effectors of immune responses. Recently earthworm protein and its coelomic fluid were reported to have cytolytic, agglutinating, proteolytic, haemolytic, mitogenic, anti-pyritic, tumorstatic and antibacterial activities vohora and khan found earthworms to have healing effect on wounds, chronic folds, piles and sore throat.
Earthworm fluid destroys lung cancer cells in lab polish scientists say their ongoing research into the potential use of earthworms' coelomic fluid to one day treat lung cancer is revealing. Collection of coelomic fluid the earthworm (mature) , perionyx excavatus is collected and identify in the molecular biology lab in jamal mohamed college, being free from harsh chemicals, this fluid does not produce any side effects, such as skin irritation this skin disorder responds quite well to this fluid. Earthworms are useful bioindicator organisms for soil biomonitoring recently the use of pollution biomarkers in earthworms has been increasingly investigated for soil monitoring and assessment earthworm coelomic fluid is particularly interesting from a toxicological perspective, because it is responsible for pollutant disposition and tissue distribution to the whole organism. Following objectives: define the normal earthworm coelomic fluid protein profile, and assess changes to the normal protein profile , resulting from exposure to an external agent, in this case, interacoelomic injection of the bacterium ( a. To evaluate the effects of time after adding coelomic fluid on the growth of hela cells, the cells were treated with 01 mg/ml of coelomic fluid for 12 h, 24 h, 48 h and 72 h, and the apoptotic rates were determined by ao/eb assay.
Coelomic fluid in an earthworm is the fluid within the coelom which serves as a circulatory medium, and locomotion this fluid also serves as a site for temporary food storage, excretion of nitrogen containing wastes, and for maturation of gametes coelom is a fluid filled cavity within the mesoderm it can be seen as the digestive track of an. The coelomic fluid of earthworm is known to contain a variety of humoral factors to combat potential pathogens that many migrate from environment into the body ,  the data mean ± sd values of body weight of the experimental animal, lampito maruitii, before and after depletion of the coelomic fluid is shown in the table 1. Earthworm coelomic fluid or blood is in the sulphate concentration the reason for this is the necessity to make up the anion deficit brought about by the relatively low chloride.
The role of the coelomic fluid in the movements of earthworms 111 extension and thrust when the circular muscles of the body wall contract against it. Context: earthworm eisenia foetida (lumbricus rubellus), a traditional chinese medicine, is used for treating many diseases, and its coelomic fluid has extensive biological functionsobjective: the hemolytic, antibacterial and antitumor activities of an earthworm protein purified from coelomic fluid were investigated in vitromaterials and methods: we used ultrafiltration, gel chromatography.
The earthworm’s body cavity contains coelomic fluid, which has several biological activities such as antibacterial, hemolytic, agglutinative, and mitogenic activities [5–9] increasing amounts of evidence indicate that earthworm extract has various beneficial pharmacological activities, including fibrinolytic and anticoagulative activities. Worms depleted of coelomic fluid and cells were injected with 100/^1 of body fluid containing a similar number of bacteria or with earthworm saline as controls in order to test mor- tality of initially normal worms as compared with those depleted of their coelomocytes. With discharge of coelomic fluid (podolak--machowska et al 2013) convulsive body movements are common in effects of prilocaine on earthworm mobility figure 2 shows concentration-dependent and time-dependent anesthetic effects of prilocaine on adult specimens of d veneta worms were com.